Cell Line Authentication Kit

Str profiling aids in the detection of misidentified cross contaminated or genetically drifted cells which invalidate research results.

Cell line authentication kit.

Standardization of str profiling guidelines using multiple kits. The idexx bioanalytics cell line authentication guide has the answers. The profiling kits are only suitable for cell lines of human origin. The test utilizes primers that pick up repeated segments of dna that are typically 2 6 base pairs in length.

Human str profiling cell authentication service short tandem repeat profiling of cell lines is an easy to use way to authenticate confirm the identity of your cells. This is the same technology used by the fbi for human dna identification testing hall explains. This service includes a collection kit dna collection card postage paid return envelope and certificate of analysis. The number of repeats at each.

Using standardized techniques authentication enables communication among all users about the resource and ensures valid reproducible experimental results. The current recommended method for cell line authentication is short tandem repeat str profiling. Grcf dna services offers cell line authentication analysis following ansi atcc asn 0002 2011 authentication of human cell lines. Further the results can vary depending on the history of the cell line and the type of media used for cell growth.

Within the past five years a handful of publications have been retracted due to invalidated use of certain cell lines. The impact of cell line contamination. Mouse cell line authentication possible contamination is detected by one mouse gender marker two human markers and a 12 plex panel including 9 mouse tetranucleotide microsatellites. Rapd assay design for the authentication of mammalian microbial and other cell lines has to be species specific.

Our authentication guide provides a road map to simplify compliance with the new standards including. Cell line authentication kits cross contaminated or misidentified cell lines can ruin experiments leading to loss of precious time and funding. Authentication of a cell line is the sum of the process by which a line s identity is verified and shown to be free of contamination from other cell lines and microbes. To show the utility of the cla identifiler plus kit and seqstudio instrument for cell line authentication we analyzed dna from cultures of five different commonly used cell lines.