Cdk2 Antibody Cell Signaling

The antibody weakly cross reacts with cdc2 phosphorylated at thr161.

Cdk2 antibody cell signaling.

Phospho cdk2 thr160 antibody detects endogenous levels of cdk2 only when phosphorylated at threonine 160. Cyclin dependent kinase 2 p33cdk2 is an important component of the cell cycle machinery. Like p34cdc2 kinase activity is regulated by phosphorylation state as well as association with a cyclin subunit and a cdk inhibitor. It does not cross react with other phosphorylated cyclin dependent kinases.

An antibody shouldn t be one of the variables in your experiment. Cyclin dependent kinase 2 p33cdk2 is an important component of the cell cycle machinery. Cdk2 knockout hap1 cell lysate 20 µg lanes 1 and 2. Ab32147 was shown to specifically react with cdk2 when cdk2 knockout samples were used.

To prepare 10 ml add 30 µl triton x 100 to 10 ml 1x pbs. Antibody dilution buffer 1x pbs 1 bsa 0 3 triton x 100. Like p34cdc2 kinase activity is regulated by phosphorylation state as well as association with a cyclin subunit and a cdk inhibitor. Kinase activity associated with cyclin a cdc2 is found only in g2 phase.

Cyclin dependent kinase 2 p33cdk2 is an important component of the cell cycle machinery. Inhibitory phosphorylation occurs on thr14 and tyr15 1. Immunoprecipitation of cdk12 from pc 3 cell extracts using normal rabbit igg 2729 lane 2 or cdk12 antibody lane 3. Like p34cdc2 kinase activity is regulated by phosphorylation state as well as association with a cyclin subunit and a cdk inhibitor.

Green signal from target ab32147 observed at 34 kda lanes 3 and 4. Mix well then add 0 1 g bsa mix. Lane 1 is 10 input. Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding thr160 of human cdk2.

Inhibitory phosphorylation occurs on thr14 and tyr15 1. Western blot analysis was performed using cdk12 antibody. Anti rabbit igg h l f ab 2 fragment alexa fluor 488 conjugate 4412. Recommended fluorochrome conjugated anti rabbit secondary antibodies.

Inhibitory phosphorylation occurs on thr14 and tyr15 1. Red signal from loading control ab8226 observed at 42 kda lanes 5 and 6. Cdk2 antibodies cdk2 antibodies p33cdk2 associates with cyclin a in human cells. Merged red and green signal.

Lanes 1 3 and 5. Cdk2 cell division protein kinase 2 cyclin dependent kinase 2 cdc2 related protein kinase cell devision kinase 2 p33 protein kinase p33 cdk2 immunogen.